Recombinant human IL-31 protein - Bioactive cytokine

Recombinant cytokine, source: E. coli

ABOUT

Human IL-31 protein - E. coli-expressed, tag-free, carrier-free

Recombinant human IL-31 is a high-quality and biologically active cytokine, validated using proprietary IL-31 reporter cells. This member of the IL-6/gp130 family is produced in E. coli and thoroughly purified to remove endotoxins.

Recombinant human IL-31 can be used together with HEK-Blue™ IL-31 cells for the screening of inhibitory molecules, such as Nemolizumab, a therapeutic monoclonal antibody targeting the IL-31Rα subunit of the IL-31 receptor (see figures).

 

Key features

  • Each lot is validated using HEK-Blue™ IL-31 cells
  • Endotoxin ≤ 0.1 EU/µg
  • 0.2 µm sterile-filtered

Applications

  • Standard for IL-31 detection and quantification assays
  • Screening and release assays for antibodies blocking IL-31 signaling
  • Screening and release assays for engineered IL-31

 

Interleukin-31 (IL-31) is a cytokine that plays a role in early protective inflammatory processes, likely regulating alarmins in damaged mucosal tissues. IL-31 is strongly involved in chronic inflammation in different skin diseases, including atopic dermatitis and allergic contact dermatitis.

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All InvivoGen products are for internal research use only, and not for human or veterinary use.

IL-31 signaling and biological functions
IL-31 signaling and biological functions

SPECIFICATIONS

Specifications

Source
E. coli
Species
Human
Accession sequence

Q6EBC2

Protein size
141 a.a. (S24-T164)
Carrier
Carrier-free
Tag
Tag-free
Purity
≥ 98% (SDS-PAGE)
Solubility

100 μg/ml in water

Formulation buffer

Phosphate buffer saline (pH 7.4)

Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (provided)
Sterility

0.2 µm filtration

Endotoxin

The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Applications

Cellular assays (tested), ELISA

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Recombinant human IL-31
  • Cat code: 
    rcyec-hil31
  • Quantity: 
    10 µg
Includes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 6 months

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

IL-31 background

The cytokine interleukin 31 (IL-31) belongs to the Type I/II cytokine receptor family, and more specifically to the IL-6/gp130 family. IL-31 signals through a heterodimeric receptor comprised of the gp130-like subunit IL-31Rα and the OSMRb (oncostatin M receptor) subunit. The binding of IL-31 to its receptor triggers activation of the JAK/STAT, Akt/PI3K, and MAPK signaling pathways [1,2]. IL-31 receptor complex is expressed in epithelial, neuronal as well as immune cells. Little is known about the physiological function of IL-31. It is mainly secreted by IL-4-activated T cells, thereby contributing to Th2-weighted inflammation [1,2]. Several reports indicate that IL-31 is implicated in disorders such as atopic dermatitis, asthma, and inflammatory bowel disease [1,2]. Of note, IL-31 appears to be a critical cytokine involved in neuro-immune communication through the mediation of inflammatory itch [1,2]. Targeting the IL-31 pathway is a promising therapeutic option for dermatologic and non-dermatologic diseases with enhanced IL-31 expression [1,2].

 

​References:

1. Bagci I.S. and Ruzicka, T., 2018. IL-31: A new player in dermatology and beyond. J. Allergy Clin Immunol. 141(3):858-866.
2. Datsi A., et al., 2021. Interleukin-31: The “itchy” cytokine in inflammation and therapy. Allergy. 76:2982-97.

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