Recombinant human TGF-β1
-
Cat.code:
rcyc-htgfb1
- Documents
ABOUT
Human TGF-β1 - Mammalian cell-expressed, tag-free, with HSA
Recombinant TGF-β1 is a high-quality and biologically active cytokine, validated using proprietary TGF-β reporter cells. This TGF-β isoform is produced in HEK293 cells to ensure protein glycosylation and bona fide 3D structure.
Recombinant human TGF-β1 can be used together with HEK-Blue™ TGF-β cells for the screening of inhibitory molecules, such as Fresolimumab, a monoclonal antibody targeting all isoforms of TGF-β (see figures).
Key features
- Each lot is validated using HEK-Blue™ TGF-β cells
- Endotoxin < 0.1 EU/µg
- 0.2 µm sterile-filtered
Applications
- TGF-β detection and quantification assays (positive control)
- Screening and release assays for antibodies blocking TGF-β signaling
- Screening and release assays for engineered TGF-β proteins
Transforming growth factor-beta (TGF-β), also known as tumor growth factor-beta, is a secreted cytokine that regulates numerous cellular functions, such as cell proliferation, apoptosis, differentiation, and migration.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
P01137
100 μg/ml in water
Phosphate buffer saline (pH 7.4), 5% saccharose, 2% HSA
0.2 µm filtration
The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
Cellular assays (tested), ELISA
Each lot is functionally tested and validated.
CONTENTS
Contents
-
Product:Recombinant human TGF-β1
-
Cat code:rcyc-htgfb1
-
Quantity:10 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Tumor growth factor-beta (TGF-β) belongs to a family of structurally related cytokines that regulate a plethora of cellular functions, such as proliferation, apoptosis, differentiation, and migration [1,2]. TGF-β exists in at least three isoforms; TGF-β1, TGF-β2, and TGF-β3. In the immune system, TGF-β1 is the predominant isoform [1]. It is produced by many cell types, including macrophages, in a latent form that is bound to two other polypeptides, latent TGF-β1 binding protein (LTBP) and latency-associated peptide (LAP). Upon cleavage of these proproteins, the mature TGF-β1 is released. This mature protein can bind its cell surface receptors and initiate signaling.
TGF-β binds to type II receptors (TβR-II) which recruit and activate type I receptors (TβR-I). The active ligand-heterotetrameric receptor complex signals through downstream transcriptional factors named Smads, including Smad2, Smad3, and Smad4.
Smad complexes translocate into the nucleus where they regulate the transcription of target genes, which contain one or more Smad binding elements (SBEs) in their promoter region [3]. Perturbations in TGF-β signaling affect immune homeostasis and tolerance, leading to inflammatory diseases and tumor immune evasion [3].
1. Travis MA. & Sheppard D., 2014. TGF-β activation and function in immunity. Annu Rev Immunol. 32:51-82.
2. Taylor AW., 2009. Review of the activation of TGF-beta in immunity. J Leukoc Biol. 85(1):29-33.
3. Battle E. & Massagué J., 2019. Transforming Growth Factor-beta Signaling in Immunity and Cancer. Immunity. 50(4):924.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
Need a CoA ?
