Recombinant human TGF-β1 protein

Recombinant human transforming growth factor-beta protein (with HSA)

Transforming growth factor-beta (TGF-β), also known as tumor growth factor-beta, is a secreted cytokine that regulates numerous cellular functions, such as cell proliferation, apoptosis, differentiation and migration[1, 2].

TGF-β exists in at least three isoforms; TGF-β1, TGF-β2 and TGF-β3. In the immune system, TGF-β1 is the predominant isoform [1].

It is produced by many cell types, including macrophages, in a latent form which is bound to two other polypeptides, latent TGF-β1 binding protein (LTBP) and latency-associated peptide (LAP).

Upon cleavage of these proproteins, the mature TGF-β1 is released. This mature protein can bind its cell surface receptors and initiate signaling.

Recombinant human TGF-β1 is intended for use in cell culture applications. It is produced in mammalian cells.

References:

1 - Travis MA. & Sheppard D., 2014. TGF-β activation and function in immunity. Annu Rev Immunol. 32:51-82.
2 - Taylor AW., 2009. Review of the activation of TGF-beta in immunity. J Leukoc Biol. 85(1):29-33.

Figures

SDS PAGE of the recombinant human (h)TGFβ1 protein. 1.5 μg of hTGF-β1, reduced or non-reduced, was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. The bands were detected at ~15 kDa (reduced condition) and ~25 kDa (non-reduced condition).

Dose-response in HEK-Blue™ TGF-β1 cells to recombinant TGF-β1 cytokine. Cells were stimulated with increasing concentrations of recombinant human TGF-β1. After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: Mammalian; HEK293 cells

UniProt ID: P01137

Formulation: Recombinant human TGF-β1 was lyophilized from a 0.2 mm filtered phosphate buffer solution (pH 7.4) containing human serum albumin (HSA).

Purity: >95%, as determined by SDS-PAGE.

Molecular mass: ~ 24 kDa in non-reducing conditions

Solubility: 100 μg/ml in water

Endotoxin level: < 0.1 EU/µg as determined by LAL assay

Bioactivity: Validated using HEK-Blue™ TGF-β cells

Contents

• 10 μg of TGFβ1 provided as a white lyophilized powder
• 1.5 ml endotoxin-free water

TGFβ1 is shipped at room temperature.

Upon receipt TGFβ1 should be stored at -20°C.