Recombinant human TSLP protein - Bioactive cytokine

Recombinant cytokine, source: CHO cells

ABOUT

Human TSLP protein - Mammalian cell-expressed, His-tag, carrier-free

Recombinant human TSLP is a high-quality and biologically active cytokine, validated using proprietary TSLP reporter cells. This IL-7-like cytokine and member of the IL-2/γc superfamily is produced in CHO cells to ensure protein glycosylation and bona fide 3D structure.

Recombinant human TSLP can be used together with HEK-Blue™ TSLP cells for the screening of inhibitory molecules, such as Tezepelumab, a monoclonal antibody targeting TSLP (see figures).

 

Key features

  • Each lot is validated using HEK-Blue™ TSLP cells
  • Endotoxin ≤ 0.1 EU/µg
  • 0.2 µm sterile-filtered

Applications

  • Standard for TSLP detection and quantification assays
  • Screening and release assays for antibodies blocking TSLP signaling
  • Screening and release assays for engineered TSLP

 

Thymic stromal lymphopoietin (TSLP) is a critical mediator of type 2 (T2) immune responses, acting at the early stages of inflammation. Abnormal TSLP expression is linked to immune-mediated inflammation diseases, notably asthma and allergic airway inflammation.

More details

All InvivoGen products are for internal research use only, and not for human or veterinary use.

TSLP signaling and biological functions
TSLP signaling and biological functions

SPECIFICATIONS

Specifications

Source
CHO cells
Species
Human
Accession sequence

Q969D9

Protein size
131 a.a. (Y29-Q159, with R127A and R130S mutations)
Molecular weight
~ 19.9 kDa (SDS-PAGE)
Carrier
Carrier-free
Tag
His6
Purity
≥ 93% (SDS-PAGE)
Solubility

100 μg/ml in water

Formulation buffer

Phosphate buffer saline (pH 7.4), 5% saccharose

Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (provided)
Sterility

0.2 µm filtration

Endotoxin

The absence of bacterial contamination (e.g. lipoproteins and endotoxins) is confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Applications

Cellular assays (tested), ELISA

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Recombinant human TSLP
  • Cat code: 
    rcyc-htslp-hs
  • Quantity: 
    10 µg
Includes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7)-like cytokine with multiple functions, including cell proliferation and survival. TSLP binds a heterodimeric transmembrane receptor comprising the shared IL-7 receptor alpha-chain (IL-7Rα, CD127) and the TLSP receptor chain (TSLPR, CRLF2), a γc-like subunit. It signals through tyrosine kinases of the Janus family (JAK1 and JAK2) and signal transducer and transcription activators (STATs), notably STAT5 [1]. TSLP is best known as a critical mediator of type 2 immune responses and a promoter of Th2 cell-mediated diseases (e.g. asthma) [1,2].

 

References:

1. Ebina-Shibuya R. & Leonard WJ., 2023. Role of thymic stromal lymphopoietin in allergy and beyond. Nat Rev Immunol, 23(1):24-37.
2. Schmitt P. et al., 2024. TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation. J Exp Med. 221(6) e20231236.

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