Recombinant human CD40L protein

Recombinant Human CD40 Ligand protein (with HSA)

CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a type II transmembrane glycoprotein belonging to the TNF family.

CD40L is predominantly expressed on activated CD4+ T lymphocytes and interacts with CD40 on antigen-presenting cells to regulate both humoral and cellular immune responses.

Recombinant human CD40L (rhCD40L) is a 149 amino acid, non-glycosylated protein. The approximate molecular mass of CD40L is 16.3 kDa. CD40L is produced in Escherichia coli.

rhCD40L is used to induce its cognate signalling pathway in HEK-Blue™ CD40L cells.

Figures

SDS PAGE of the recombinant human (h)CD40L protein. 1.5 μg of hCD40L was loaded on a 12% Mini-PROTEAN® TGX StainFree™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. A band was detected at ~16 kDa.

Dose-response in HEK-Blue™ CD40 cells to recombinant hCD40L protein. Cells were stimulated with increasing concentrations of recombinant human (h)CD40L. After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: E. coli

Uniprot ID: P29965

Formulation: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.0) with human serum albumin (HSA) as a carrier protein

Purity: >95%, by SDS-PAGE

Molecular mass: ~ 16 kDa

Solubility: 100 μg/ml in water

Endotoxin level: < 0.1 EU/μg protein as determined by the LAL method

Bioactivity: Validated using HEK-Blue CD40L cells

Contents

Recombinant human CD40L is provided as a white lyophilized powder.

• 10 μg of recombinant human CD40L
• 1.5 ml endotoxin-free water.

Recombinant human CD40L is shipped at room temperature.

Upon receipt it should be stored at -20 °C.