CD40L Reporter HEK 293 Cells
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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HEK-Blue™ CD40L cells Human CD40L SEAP Reporter Cells |
Show product |
3-7 x 10e6 cells |
hkb-cd40
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HEK-Blue™ CD40L vial Additional cell vial |
Show product |
3-7 x 10e6 cells |
hkb-cd40-av
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Notification: Reference #hkb-cd40-av can only be ordered together with reference #hkb-cd40.
CD40L Reporter Cells
HEK-Blue™ CD40L Cells signaling pathway
HEK-Blue™ CD40L cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive CD40 ligand (CD40L) by monitoring the activation of the NF-κB pathway. CD40L is a protein that is primarily expressed on activated T cells and is a member of the tumor necrosis factor superfamily. Of note, CD40L, together with its receptor CD40, plays a pivotal role in cellular and humoral immunity [1-3].
Cell line description:
HEK-Blue™ CD40L cells were generated by stable expression of the gene encoding for human CD40 (aka Bp50) and an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. The binding of CD40L to its receptor triggers a signaling cascade leading to the activation of NF-κB and the subsequent production of SEAP. This can be readily assessed in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent.
HEK-Blue™ CD40L cells detect human and murine CD40L (see figures). Of note, HEK293 cells endogenously express the receptors for the cytokines IL-1β and TNF-α which share a common signaling pathway with CD40L. Consequently, HEK-Blue™ CD40L cells also respond to IL-1β and TNF-α (see figures). However, the IL-1β- and TNF-α-mediated SEAP production can be blocked using neutralizing antibodies, such as anti-hIL-1β-IgG and anti-hTNF-α-IgA2 respectively.
Key features:
- Fully functional CD40L signaling pathway
- Readily assessable NF-κB-inducible SEAP reporter activity
Applications:
- Detection of human and murine CD40L
- Screening of anti-CD40 and anti-CD40L antibodies
References:
1. Karnell J.L. et al., 2019. Targeting the CD40-CD40L pathway in autoimmune diseases: Humoral immunity and beyond. Adv Drug Deliv Rev. 141:92-103.
2. Laman J.D. et al., 2017. Functions of CD40 and Its Ligand, gp39 (CD40L). Crit Rev Immunol. 37(2-6):371-420.
3. Elgueta R. et al., 2009. Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol. Rev. 229, 152–172.
Specifications
Antibiotic resistance: Blasticidin, Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 100 µg/ml Normocin™
Guaranteed mycoplasma-free
Specification: human and murine CD40L
Detection range:
- 3 ng - 1 μg/ml for human CD40L
- 3 ng - 1 μg/ml for murine CD40L
This product is covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
- 1 vial containing 3-7 x 106 cells
- 1 ml of Blasticidin (10 mg/ml)
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Back to the topDetails
CD40 Ligand (CD40L), also known as CD154, TRAP, or gp39, is a type II transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) family. It is mainly expressed in CD4+-T cells and interacts with CD40 on antigen-presenting cells to regulate both humoral and cellular immune responses [1-3].
The CD40 cytoplasmic domain binds directly to several TNF receptor-associated factors (TRAFs), and this interaction is thought to initiate CD40 signaling. CD40-mediated signaling results in NF-κB, c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) activation. CD40L-CD40 interactions are thought to play an important role in the pathogenesis of many diseases [4, 5].
1. Karnell J.L. et al., 2019. Targeting the CD40-CD40L pathway in autoimmune diseases: Humoral immunity and beyond. Adv Drug Deliv Rev. 141:92-103.
2. Laman J.D. et al., 2017. Functions of CD40 and Its Ligand, gp39 (CD40L). Crit Rev Immunol. 37(2-6):371-420.
3. Elgueta R. et al., 2009. Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol. Rev. 229, 152–172.
4. Seijkens T. et al., 2013. CD40–CD40L: Linking pancreatic, adipose tissue and vascular inflammation in type 2 diabetes and its complications. Diabetes and Vascular Disease Research. 10: 115 - 122.
5. Daoussis D. et al., 2004. Targeting CD40L: a promising therapeutic approach. Clin Diagn Lab Immunol. 11(4):635-41.