Recombinant human IL-36 alpha protein - Bioactive cytokine

Recombinant cytokine, source: E. coli

ABOUT

Human IL-36α protein - E. coli-expressed, tag-free, carrier-free

Recombinant human IL-36α is a high-quality and biologically active cytokine, validated using proprietary IL-36 reporter cells. This member of the IL-1/TLR cytokine superfamily is produced in E. coli and thoroughly purified to remove endotoxins.

Recombinant human IL-36α can be used together with HEK-Blue™ IL-36 cells for the screening of inhibitory molecules, such as Imsidolimab,a therapeutic monoclonal antibody targeting the IL-36 receptor (see figures).

 

Key features

  • Each lot is validated using HEK-Blue™ IL-36 cells
  • Endotoxin ≤ 1 EU/µg (LAL assay)
  • 0.2 µm sterile-filtered

Applications

  • Standard for IL-36α detection and quantification assays
  • Screening and release assays for antibodies blocking IL-36 signaling
  • Screening and release assays for engineered IL-36α

 

Interleukin-36 alpha (IL-36α) belongs to the IL-1 superfamily and is one of the three pro-inflammatory isoforms of IL-36. Dysregulation of IL-36 isoform expression and signaling has been associated with inflammatory diseases such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease.

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All products are for internal research use only, and not for human or veterinary use.

IL-36 signaling and biological functions
IL-36 signaling and biological functions

SPECIFICATIONS

Specifications

Source
E. coli
Species
Human
Synonyms
FIL1 epsilon
Interleukin-1 epsilon
Interleukin-1 family member 6 (IL-1F6)
Accession sequence

Q9UHA7

Protein size
153
Molecular weight
~ 15.6 kDa (SDS-PAGE)
Carrier
Carrier-free
Tag
Tag-free
Purity
≥95% (SDS-PAGE)
Solubility

100 μg/ml in water

Formulation buffer

Phosphate buffer saline (pH 7.4), 6% trehalose, 5 mM EDTA, 0.05% Tween 80

Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (provided)
Endotoxin

The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Tested applications

Cellular assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Recombinant human IL-36α
  • Cat code: 
    rcyec-hil36a
  • Quantity: 
    10 µg
Includes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Interleukin-36 background

The cytokine interleukin 36 (IL-36) belongs to the IL-1 superfamily. Three isoforms, IL-36α, IL-36β, and IL-36γ, mediate pro-inflammatory functions, while a fourth one, IL-36Ra, acts as an antagonist [1,2]. IL-36 signalization requires the formation of a complex comprised of two subunits, IL-1R6 (aka IL-36R) and IL-1R3 (aka IL-1RAcP, IL-1 receptor accessory protein). The binding of agonist ligands to the IL-36R allows the recruitment of IL-1RAcP and the production of pro-inflammatory cytokines and chemokines through the activation of NF-κB and AP-1 [1,2]. The IL-36Ra antagonist inhibits the signaling by binding to IL-36R and preventing the recruitment of IL-1RAcP [1,2]. IL-36-associated immune response mainly takes place in barrier tissues, such as the skin, lungs, and intestines. Dysregulation of IL-36 isoform expression and signaling has been associated with inflammatory diseases such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease [1,2].

 

1. Buhl A-L. & Wenzel J., 2019. Interleukin-36 in infectious and inflammatory skin diseases. Front. Immunol. 10(1162). doi: 10.3389/fimmu.2019.01162.
2. Zhou L. & Todorovic V., 2021. Interleukin-36: Structure, Signaling and Function. Protein Reviews: Volume 21. doi: 10.1007/5584_2020_488.

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