Human IgG4 (S228P) Isotype Control Antibody

E. coli β-Galactosidase antibody (clone T9C6) - Recombinant

ABOUT

Monoclonal human IgG4 (S228P) antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG4 (S228P) features the constant region of the human IgG4 (S228P) isotype and the variable region of Mouse IgG2a Control.

Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal).

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice. Anti-β-Gal-hIgG4 (S228P) can be used as an isotype control for human IgG4 (S228P) antibodies.

It contains an engineered hinge region mutation (S228P) designed to prevent exchange of IgG4 molecules.

Anti-β-Gal-hIgG4 (S228P) was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity  chromatography.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Negative control for human IgG4 (S228P) antibodies

Isotype
hIgG4 (S228P)
Source
CHO cells
Purification
Affinity chromatography
Formulation buffer

Sodium phosphate buffer with glycine, saccharose and stabilizing agents

Preservative
Azide-free
Purity
Purified by affinity chromatography with protein G
Reconstitution buffer
Sterile water
Tested applications

Negative control for human IgG4 (S228P) antibodies

Quality control

The absence of binding of Anti-β-Gal-hIgG4 (S228P) to human cell lines has been controlled using flow cytometry. The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

CONTENTS

Contents

  • Product: 
    Anti-β-Gal-hIgG4 (S228P)
  • Cat code: 
    bgal-mab114
  • Quantity: 
    200 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Reconstituted antibody is stable for 1 month when stored at 4°C and for 1 year when aliquoted and stored at -20°C.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-β-Gal-hIgG4 (S228P)

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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