Anti-β-Gal-hIgG4 (S228P)

Monoclonal human IgG4 (S228P) antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG4 (S228P) features the constant region of the human IgG4 (S228P) isotype and the variable region of Mouse IgG2a Control.

Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal).

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice. Anti-β-Gal-hIgG4 (S228P) can be used as an isotype control for human IgG4 (S228P) antibodies.

It contains an engineered hinge region mutation (S228P) designed to prevent exchange of IgG4 molecules.

Anti-β-Gal-hIgG4 (S228P) was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity  chromatography.

Specifications

Clonality: Monoclonal antibody

Specificity: Targets cells expressing E. coli β-galactosidase (β-Gal)

Isotype: Human IgG4 (S228P), kappa

Source: CHO cells

Application: Negative control for human IgG4 (S228P) antibodies

Quality control:

• The absence of binding of Anti-β-Gal-hIgG4 (S228P) to human cell lines has been controlled using flow cytometry.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

Contents

Anti-β-Gal-hIgG4 (S228P) purified monoclonal antibody is provided azide-free and lyophilized. It is available in two quantities:

• bgal-mab114: 200 µg
• bgal-mab114-1: 5 x 200 µg (1 mg)

Product is shipped at room temperature.

 Upon receipt, store lyophilized antibody at -20 °C.