Human IgG1fut Isotype Control Antibody

Monoclonal non-fucosylated hIgG1 antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG1fut features the constant region of the human IgG1 isotype and the variable region of Mouse IgG2a Control. Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal).

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice.

Anti-β-Gal-hIgG1fut is a non-fucosylated antibody. The absence of the fucose residue from the N-glycans of IgG-Fc results in dramatic enhancement of antibody-dependent cellular cytotoxicity (ADCC) without any detectable change in complement-dependent cytotoxicity (CDC) or antigen binding capability [1,2].

Anti-β-Gal-hIgG1fut can be used as an isotype control for human IgG1fut antibodies.

Anti-β-Gal-hIgG1fut was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography.

References:

1. Yamane-Ohnuki N. & Satoh M., 2009. Production of therapeutic antibodies with controlled fucosylation.corresponding MAbs. 1(3): 230–236.
2. Mizushima T., 2011. Structural basisfor improved efficacy of therapeutic antibodies on defucosylation of their Fc glycans. Genes Cells. 16(11): 1071–1080.