Anti-β-Gal-hIgG2

Monoclonal human IgG2 antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG2 features the constant region of the human IgG2 isotype and the variable region of Mouse IgG2a Control. Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal).

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice. It can be used as an isotype control for human IgG2 antibodies.

Human IgG2 is the second most common antibody present in serum. Human IgG2 is resistant to cleavage by proteolytic enzymes, due to a short hinge region.

Human IgG2 displays low complement-dependent cytotoxicity (CDC) and very low antibody-dependent cell-mediated cytotoxicity (ADCC).

Anti-β-Gal-hIgG2 was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity  chromatography.

Specifications

Clonality: Monoclonal antibody

Specificity: Targets cells expressing E. coli β-galactosidase (β-Gal)

Isotype: Human IgG2, kappa

Application: Negative control for human IgG2 antibodies

Quality control:

• The absence of binding of Anti-β-Gal-hIgG2 to human cell lines has been controlled using flow cytometry.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

Contents

Anti-β-Gal-hIgG2 purified monoclonal antibody is provided azide-free and lyophilized. It is available in two quantities:

• bgal-mab2: 200 µg
• bgal-mab2-1: 5 x 200 µg (1 mg)

Product is shipped at room temperature.

Upon receipt, store at -20°C.