Human IgG1 (N298A) Control Antibody

E. coli β-Galactosidase antibody (clone T9C6) - Recombinant

ABOUT

Monoclonal human IgG1 (N298A) antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG1 (N298A) features the constant region of the human IgG1 (N298A) isotype and the variable region targeting E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice.

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice.

Anti-β-Gal-hIgG1 (N298A) can be used as an isotype control for human IgG1 antibodies containing the N298A mutation. Antibodies with this mutation contain a modified Fc region designed to limit antibody-dependent cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) [1, 2], as this mutation eliminates their ability to bind to human Fcγ receptors.

Anti-β-Gal-hIgG1 (N298A) was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography with protein G.

 

References:

1. Spigel D. et al., 2013. Clinical activity, safety, and biomarkers of MPDL3280A, an engineered PD-L1 antibody in patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) [ASCO abstract 8008]. J Clin Oncol. 31(15)(suppl).
2. Herbst RS. et al., 2014. Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients. Nature. 515(7528):563-7.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

E. coli β-galactosidase (β-Gal)

Applications

Negative control for human IgG1 (N298A) antibodies

Species
Human
Isotype
hIgG1 (N298A)
kappa
Clone
T9C6
Tag
Tag-free
Source
CHO cells
Purification
Protein G
Formulation buffer

Sodium phosphate buffer with glycine, saccharose and stabilizing agents

Preservative
Azide-free
Reconstitution buffer
Sterile water (not provided)
Tested applications

Cellular assay, flow cytometry, ELISA

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-β-Gal-hIgG1 (N298A)
  • Cat code: 
    bgal-mab12
  • Quantity: 
    200 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-β-Gal-hIgG1 (N298A)

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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