
Anti-β-Gal-hIgG1 (N298A)
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Cat.code:
bgal-mab12
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ABOUT
Monoclonal human IgG1 (N298A) antibody against E. coli β-galactosidase
Anti-β-Gal-hIgG1 (N298A) features the constant region of the human IgG1 (N298A) isotype and the variable region targeting E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice.
This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice.
Anti-β-Gal-hIgG1 (N298A) can be used as an isotype control for human IgG1 antibodies containing the N298A mutation. Antibodies with this mutation contain a modified Fc region designed to limit antibody-dependent cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) [1, 2], as this mutation eliminates their ability to bind to human Fcγ receptors.
Anti-β-Gal-hIgG1 (N298A) was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography with protein G.
References:
1. Spigel D. et al., 2013. Clinical activity, safety, and biomarkers of MPDL3280A, an engineered PD-L1 antibody in patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) [ASCO abstract 8008]. J Clin Oncol. 31(15)(suppl).
2. Herbst RS. et al., 2014. Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients. Nature. 515(7528):563-7.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
E. coli β-galactosidase (β-Gal)
Negative control for human IgG1 (N298A) antibodies
Sodium phosphate buffer with glycine, saccharose and stabilizing agents
Cellular assay, flow cytometry, ELISA
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Anti-β-Gal-hIgG1 (N298A)
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Cat code:bgal-mab12
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Quantity:200 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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