293/TLR3

HEK293 clones expressing TLR3

293/TLR3 cells were obtained by stable transfection of HEK293 cells with a pUNO-TLR3 plasmid which expresses the human or murine TLR3 gene.

The control cell line of 293/TLR3 cells is 293/null.

Figures

293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

Specifications

Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

These products are covered by a Limited Use License (See Terms and Conditions).

Contents

• 1 vial containing 3-7 x 10⁶ cells
• 100 μl Blasticidin (10 mg/ml)
• 1 ml Normocin™ (50 mg/ml)

Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

Description

TLR3 recognizes double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Stimulation with poly(I:C), a synthetic analog of dsRNA, was shown to induce hyporesponsiveness in TLR3-deficient mice and considerable responsiveness in HEK293 cells expressing TLR3 [1], suggesting a specific recognition to poly(I:C) by TLR3. TLR3 signals mainly through a MyD88-independent pathway involving the TRIF/TICAM1 adapter protein that leads to the production of IFN-ß and causes dendritic cells to mature [2].

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature, 413(6857):732-8.
2. Yamamoto m. et al. 2002. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-ß promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672.

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