293/Control Cells

HEK293 Control cells

293/Control cells were generated by stably transfecting HEK293 cells with a pUNO control plasmid expressing either the lacZ reporter gene or a multiple cloning site.

• 293/LacZ cells express the lacZ reporter gene and can be used as a control cell line for 293/TLR and 293/NOD cells.
• 293/null cells contain a multiple cloning site and can be used as a control cell line for 293/TLR and 293/NOD cells.
• 293XL/null cells express the human antiapoptotic Bcl-XL gene and contain a multiple cloning site. They can be used as a control cell line for 293XL/TLR cells.

Figures

293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

Specifications

Antibiotic resistance: Blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

This product is covered by a Limited Use License (See Terms and Conditions).

Contents

• 1 vial containing 3-7 x 10⁶ cells
• 100 μl Blasticidin (10 mg/ml)
• 1 ml Normocin™ (50 mg/ml)

Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

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