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293/Control Cells

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293/LacZ

LacZ expressing 293 cell line

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3-7 x 10e6 cells

293-lacz

293/null

293 Control cell line

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3-7 x 10e6 cells

293-null

293XL/null

293XL Control cell line

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3-7 x 10e6 cells

293xl-null

HEK293 Control cells

293/Control cells were generated by stably transfecting HEK293 cells with a pUNO control plasmid expressing either the lacZ reporter gene or a multiple cloning site.

  • 293/LacZ cells express the lacZ reporter gene and can be used as a control cell line for 293/TLR and 293/NOD cells.
  • 293/null cells contain a multiple cloning site and can be used as a control cell line for 293/TLR and 293/NOD cells.
  • 293XL/null cells express the human antiapoptotic Bcl-XL gene and contain a multiple cloning site. They can be used as a control cell line for 293XL/TLR cells.

Figures

TLR and NOD induction profile of 293 clones
TLR and NOD induction profile of 293 clones

293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

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Specifications

Antibiotic resistance: Blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

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Contents

dry ice Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

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FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

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Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

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