293/NOD1

HEK293 clones expressing NOD1

293/NOD1 cells were obtained by stable transfection of HEK293 cells with the pUNO-hNOD1 plasmid which expresses the human NOD1 gene or with the pUNO-mNOD1 plasmid which expresses the murine NOD1 gene.

The control cell line for 293/NOD1 cells is 293-null cells.

Figures

293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

293/hNOD1 cells transiently transfected with the NF-κB-inducible SEAP reporter plasmid, pNiFty-SEAP, were incubated with the NOD1 ligands, iE-DAP, Tri-DAP, and C12-iE-DAP, and the NOD2 ligands, MDP and murabutide, at various concentrations. After 24h incubation, NOD1 stimulation was assessed by measuring the levels of SEAP secreted in the supernatant by using HEK-Blue™ Detection medium, which turns blue in the presence of SEAP.

Specifications

Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Contents

• 1 vial containing 3-7 x 10⁶ cells
• 100 μl Blasticidin (10 mg/ml)
• 1 ml Normocin™ (50 mg/ml)

Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

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