293/TLR2

HEK293 clones expressing TLR2

293/TLR2 cells were obtained by stable transfection of HEK293 cells with a pUNO-TLR2 plasmid which expresses the human or murine TLR2 gene.

The control cell line for 293/hTLR2 and 293/mTLR2 cells is 293/null.

Figures

293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

Specifications

Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

These products are covered by a Limited Use License (See Terms and Conditions).

Contents

• 1 vial containing 3-7 x 10⁶ cells
• 100 μl Blasticidin (10 mg/ml)
• 1 ml Normocin™ (50 mg/ml)

Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

Details

TLR and NOD induction profile of 293 clones :
293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, aSEAP-detection medium.

FAQ Cell Lines

Any questions about our cell lines ? Visit our frequently asked questions page