pNiFty3-Lucia
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pNiFty3-Lucia pNiFty3 - mIFN-β promoter - ZeocinR - Lucia |
Show product |
20 µg |
pnf3-lc1
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Inducible Lucia luciferase reporter plasmid - mIFN-β promoter - ZeocinR
pNiFty3-Lucia plasmid is composed of two key elements: the mouse interferon-beta minimal promoter and the secreted Lucia luciferase reporter gene.
pNiFty3-SEAP plasmid is selectable with Zeocin™ in both E. coli and mammalian cells, and can be used to generate stable clones.
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Minimal Promoter: mouse IFNβ promoter
Selection: Zeocin™
Reporter Gene: Lucia luciferase
These products are covered by a Limited Use License (See Terms and Conditions).
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- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature.
Description
Minimal promoter
The proximal promoters are shorter than 500 bp and contain transcription factor binding sites. Upon stimulation in 293 cells, their expression level remains undetectable. With the addition of repeated TFBS, the proximal promoters become inducible by the appropriate stimulus and drive the expression of the reporter gene.
IFN-β promoter: the mouse IFN-β minimal promoter comprises several positive regulatory domains that bind different cooperating transcription factors such as NF-kB, IRF3 and IRF7 [1].
Reporter Gene
Lucia luciferase reporter gene: Lucia luciferase is a secreted luciferase with strong bioluminescent activity
The Lucia reporter gene is ideal for promoter activity and gene expression studies. Lucia is encoded by a synthetic gene derived by genetic engineering of copepod luciferase genes. Lucia luciferase activity can be detected and quantified directly in the culture medium of transfected cells using InvivoGen's QUANTI-Luc™ detection reagent.
1. Vodjdani G. et al., 1988. Structure and characterization of a murine chromosomal fragment containing the interferon beta gene. J Mol Biol. 204(2):221-31.
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