pFUSEN-Lucia-mG2aFc

Lucia luciferase-tagged Fc-fusion protein expression plasmid - Mouse IgG2a

ABOUT

Production of Lucia luciferase-tagged IgG2a-Fc fusion proteins

pFUSEN-Lucia-mG2aFc is a cloning plasmid for the generation of a Lucia® luciferase-tagged Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG2a heavy chain and the hinge region fused to the N-terminus of a protein of interest.

Lucia® luciferase is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins.

It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.

Mouse IgG2a-Fc is the murine equivalent of human IgG1-Fc. It has moderate to high affinity for FcγRs and complement receptors, inducing strong ADCC and CDC.

Disclaimer:  This product is covered by a Limited Use License (See Terms and Conditions). 
Lucia® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents.
All InvivoGen products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Fc-fusion protein generation

Tag
Lucia®, N-ter
Plasmid backbone
pFUSEN
Antibiotic resistance
Zeocin®
Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile water
Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pFUSEN-Lucia-mG2aFc
  • Cat code: 
    pfcn-lcmg2a
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pFUSEN-Lucia-mG2aFc

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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