pFUSE-Lucia-mG2a-Fc

Lucia luciferase-tagged Fc-fusion expression plasmid - Mouse IgG2a

ABOUT

Production of Lucia luciferase-Tagged IgG2a-Fc fusion proteins

pFUSE-Lucia-mG2a-Fc plasmid allows the production of  Lucia luciferase-Tagged mouse IgG2a-Fc fusion proteins. This plasmid can be used to make recombinant Lucia luciferase-Tagged murine IgG2a-Fc fusion proteins.

Lucia is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins. It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.

Quantification of Lucia-Fc expression can be determined using QUANTI-Luc™.

 

These products are covered by a Limited Use License (See Terms and Conditions).

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

In vitro transfection, Fc-fusion protein production

Gene
Lucia (secreted luciferase)-MCS
Plasmid backbone
pFUSE
Antibiotic resistance
Zeocin®
Gene promoter
Human EF1α/HTLV hybrid promoter
Reporter gene
Lucia®
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Tested applications

In vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

Additional information

Fc-Region: Native, no intron

CONTENTS

Contents

  • Product: 
    pFUSE-Lucia-mG2a-Fc
  • Cat code: 
    pfuse-mg2alc
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml) 

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store product at -20 °C.
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

DOCUMENTS

Documents

pFUSE-Lucia-mG2a-Fc

Technical Data Sheet

Safety Data Sheet

Plasmid Sequence

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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