pFUSE-CHIg-rG (rabbit)

Heavy chain expression plasmid - Rabbit IgG

ABOUT

Rabbit IgG heavy chain constant region

 

pFUSE-CHIg-rG is a cloning plasmid that expresses the constant region of the rabbit IgG heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-rG additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

 

Key features of pFUSE-CHIg-rG

  • Encodes the rabbit IgG CH constant region
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin™ in both bacterial and mammalian cells
  • Available with or without the IL-2 signal sequence

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Gene
Rabbit IgHG (IgG heavy chain constant region)
Plasmid backbone
pFUSE
Antibiotic resistance
Zeocin®
Gene promoter
hEF1-HTLV prom
Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile water
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pFUSE-CHIg-rG
  • Cat code: 
    pfuse-rchg
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Resuspended DNA should be stored at -20˚C and is stable up to 1 year.

DOCUMENTS

Documents

pFUSE-CHIg-rG

Technical Data Sheet

Safety Data Sheet

Plasmid Sequence

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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