pFUSE-CHIg-mG2b
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Cat.code:
pfuse-mchg2b
- Documents
ABOUT
Murine IgG2b heavy chain constant region
pFUSE-CHIg-mG2b is a cloning plasmid that expresses the constant region of the mouse IgG2b heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-mG2b additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-mG2b
- Encodes the murine IgG2b CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin™ in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
in vitro transfection
Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.
CONTENTS
Contents
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Product:pFUSE-CHIg-mG2b
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Cat code:pfuse-mchg2b
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Plasmid Sequence
Certificate of analysis
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