pFUSE-CHIg-hG2
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Cat.code:
pfuse-hchg2
- Documents
ABOUT
Human IgG2 heavy chain constant region
pFUSE-CHIg-hG2 is a cloning plasmid that expresses the constant region of the human IgG2 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-hG2
- Encodes the human IgG2 CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin® in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Antibody generation and isotype switching
in vitro transfection
Plasmid construct has been confirmed by restriction analysis and sequencing.
CONTENTS
Contents
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Product:pFUSE-CHIg-hG2
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Cat code:pfuse-hchg2
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Plasmid Sequence
Certificate of analysis
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