pFUSE-CHIg-hG1e7

pFUSE-CHIg general plasmid map

Human IgG1 heavy chain constant region with increased CDC

pFUSE-CHIg-hG1e7 is a cloning plasmid that expresses the human IgG1 heavy chain constant region mutated K326W / E333S. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e7 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pFUSE-CHIg-hG1e7

• Encodes the human IgG1e7 CH constant region (K326W / E333S mutations)
• Long-lasting expression due to the composite hEF1-HTLV promoter
• Selectable with Zeocin™ in both bacterial and mammalian cells
• Available with or without the IL-2 signal sequence

   

Reference:

1.Idusogie EE. et al., 2001. Engineered antibodies with increased activity to recruit complement. J Immunol. 166(4):2571-5.

Specifications

• pFUSE-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain with K326W / E333S mutations

• pFUSEss-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain with K326W / E333S mutations and the IL2 signal sequence

Characteristics of K326W / E333S mutations:
- Increased binding to C1q
- Increased CDC

Both plasmids are selectable with Zeocin™.

pFUSE Contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin® (100 mg/ml)

Product is shipped at room temperature