pFUSE-CHIg-hG1e6

pFUSE-CHIg general plasmid map

Human IgG1 heavy chain constant region with increased binding to FcRn

pFUSE-CHIg-hG1e6 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with P257I + Q311I mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e6 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pFUSE-CHIg-hG1e6

• Encodes the human IgG1e6 CH constant region (P257I + Q311I mutations)
• Long-lasting expression due to the composite hEF1-HTLV promoter
• Selectable with Zeocin™ in both bacterial and mammalian cells
• Available with or without the IL-2 signal sequence

Reference:

1. Datta-Mannan A. et al., 2007. Humanized IgG1 Variants with Differential Binding Properties to the Neonatal Fc Receptor: Relationship to Pharmacokinetics in Mice and Primates. Drug Metab. Dispos. 35: 86 - 94.

Specifications

• pFUSE-CHIg-hG1e6 features the constant region of the human IgG1 heavy chain with P257I / Q311I mutations

• pFUSEss-CHIg-hG1e6 features the constant region of the human IgG1 heavy chain with P257I / Q311I mutations and the IL2 signal sequence

Characteristics of P257I / Q311I mutations:
- Increased binding to FcRn
- Unchanged half-life

Both plasmids are selectable with Zeocin™.

pFUSE Contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin® (100 mg/ml)

Product is shipped at room temperature