pFUSE-CHIg-hE
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Cat.code:
pfuse-hche2
- Documents
ABOUT
Human IgE heavy chain constant region
pFUSE-CHIg-hE is a cloning plasmid that expresses the constant region of the human IgE heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hE additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-hE
- Encodes the human IgE CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin® in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Plasmid construct has been confirmed by restriction analysis and sequencing.
CONTENTS
Contents
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Product:pFUSE-CHIg-hE
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Cat code:pfuse-hche2
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Quantity:20 µg
1 ml of Zeocin™ (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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