pFUSE-CHIg-dG1

Heavy chain expression plasmid - Dog IgG1

ABOUT

Canine IgG1 heavy chain constant region

 

pFUSE-CHIg-dG1 is a cloning plasmid that expresses the constant region of the canine IgG1 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

 

Key features of pFUSE-CHIg-dG1

  • Encodes the canine IgG1 CH constant region
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin™ in both bacterial and mammalian cells

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

ORF size
4427 bp
Plasmid backbone
pFUSE
Antibiotic resistance
Zeocin®
Subclone start
AgeI
Subclone end
NheI
Reconstitution buffer
Endotoxin-free water (not provided)
Tested applications

in vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pFUSE-CHIg-dG1
  • Cat code: 
    pfuse-dchg1
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

DOCUMENTS

Documents

pFUSE-CHIg-dG1

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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