pDRIVE-NSE / RU5′

NSE / RU5′ promoter (Mature neurons) in pDRIVE expression plasmid

Neuron-specific enolase, an isoenzyme form of enolase, occurs in mature neurons and paraneurons.

Transgenic mouse studies have shown that a 1.8 kb fragment of the rat NSE promoter is able to express a heterologous gene exclusively in postmitotic neurons and neuro-endocrine cells in parallel with endogenous NSE [1].

The NSE promoter contains a TATA-like sequence, no CAAT box and sequences for the AP-1 binding motif, AP-2 binding element, SP-1 binding sequence and cAMP response element [2].

The NSE promoter was used to create transgenic mice exhibiting the neuropathological phenotypes of Alzheimer's disease [3].

References:

1. Forss-Petter S. et al. 1990. Transgenic mice expressing beta-galactosidase in mature neurons under neuron-specific enolase promoter control. Neuron 5(2):187-97.
2. Sakimura K. et al. 1995. Upstream and intron regulatory regions for expression of the rat neuron-specific enolase gene. Brain Res Mol B 28(1):19-28.
3. Hwang DY. et al., 2004. Aberrant expressions of pathogenic phenotype in Alzheimer's diseased transgenic mice carrying NSE-controlled APPsw. Exp Neurol. 186(1):20-32.

Specifications

pDRIVE bearing rat NSE / RU5' promoter - LacZ, SEAP or Lucia

Gene Promoter: NSE : Neuron-Specific Enolase

Specificity: Mature neurons

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin™ (100 mg/ml)

Product is shipped at room temperature

Details