pDRIVE-NSE / RU5′
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
|
pDRIVE-rNSE-RU5' pDRIVE bearing rat NSE / RU5' promoter - LacZ |
Show product |
20 µg |
pdrive-rnseru5
|
|
||
|
pDRIVE5SEAP-rNSE-RU5' pDRIVE bearing rat NSE / RU5' promoter - SEAP |
Show product |
20 µg |
pdrive5s-rnseru5
|
|
||
|
pDRIVE5Lucia-rNSE-RU5' pDRIVE bearing rat NSE / RU5' promoter - Lucia |
Show product |
20 µg |
pdrive5lc-rnseru5
|
|
NSE / RU5′ promoter (Mature neurons) in pDRIVE expression plasmid
Neuron-specific enolase, an isoenzyme form of enolase, occurs in mature neurons and paraneurons.
Transgenic mouse studies have shown that a 1.8 kb fragment of the rat NSE promoter is able to express a heterologous gene exclusively in postmitotic neurons and neuro-endocrine cells in parallel with endogenous NSE [1].
The NSE promoter contains a TATA-like sequence, no CAAT box and sequences for the AP-1 binding motif, AP-2 binding element, SP-1 binding sequence and cAMP response element [2].
The NSE promoter was used to create transgenic mice exhibiting the neuropathological phenotypes of Alzheimer's disease [3].
References:
1. Forss-Petter S. et al. 1990. Transgenic mice expressing beta-galactosidase in mature neurons under neuron-specific enolase promoter control. Neuron 5(2):187-97.
2. Sakimura K. et al. 1995. Upstream and intron regulatory regions for expression of the rat neuron-specific enolase gene. Brain Res Mol B 28(1):19-28.
3. Hwang DY. et al., 2004. Aberrant expressions of pathogenic phenotype in Alzheimer's diseased transgenic mice carrying NSE-controlled APPsw. Exp Neurol. 186(1):20-32.
Specifications
pDRIVE bearing rat NSE / RU5' promoter - LacZ, SEAP or Lucia
Gene Promoter: NSE : Neuron-Specific Enolase
Specificity: Mature neurons
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
