pDRIVE-eIF4A1
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-heIF4A1 pDRIVE bearing human eIF4A1 promoter - LacZ |
Show product |
20 µg |
pdrive-heif4a1
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pDRIVE-meIF4A1 pDRIVE bearing mouse eIF4A1 promoter - LacZ |
Show product |
20 µg |
pdrive-meif4a1
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pDRIVE5SEAP-heIF4A1 pDRIVE bearing human eIF4A1 promoter - SEAP |
Show product |
20 µg |
pdrive5s-heif4a1
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pDRIVE5SEAP-meIF4A1 pDRIVE bearing mouse eIF4A1 promoter - SEAP |
Show product |
20 µg |
pdrive5s-meif4a1
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pDRIVE5Lucia-heIF4A1 pDRIVE bearing human eIF4A1 promoter - Lucia |
Show product |
20 µg |
pdrive5lc-heif4a1
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pDRIVE5Lucia-meIF4A1 pDRIVE bearing mouse eIF4A1 promoter - Lucia |
Show product |
20 µg |
pdrive5lc-meif4a1
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eIF4A1 promoter (Ubiquitous) in pDRIVE expression plasmid
The eIF-4A1 gene encoding eukaryotic initiation factor 4A is ubiquitously expressed in eukaryotic cells. The minimal promoter of the eIF-4A1 gene consists of approximately 300 bp, 80% of which are identical with those in mouse eIF-4A1 promoter.
The minimal promoter contains TATA and CAAT motifs and consensus sequences binding to SP1 and AP2 [1]. The eIF-4A1 promoter is a versatile promoter giving high-levels of expression.
In some cell lines, such as macrophages, levels of expression are higher than that obtained with the CMV promoter [2].
References:
1. Kukimoto I. et al. 1997. Characterization of the cloned promoter of the human initiation factor 4AI gene. Biochem Biophys Res Commun 233(3):844-7.
2. Quinn CM. et al. 1999. The human eukaryotic initiation factor 4AI gene (EIF4A1) contains multiple regulatory elements that direct high-level reporter gene expression in mammalian cell lines. Genomics 62(3):468-76.
Specifications
pDRIVE bearing human eIF4A1 promoter - LacZ, SEAP or Lucia
Gene Promoter: Eukaryotic Initiation Factor 4A1
Specificity: Ubiquitous
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
pDRIVE bearing mouse eIF4A1 promoter - LacZ, SEAP or Lucia
Gene: Eukaryotic Initiation Factor 4A1
Specificity: Ubiquitous
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
