pDRIVE-β-Act

β-Act promoter (Ubiquitous) in pDRIVE expression plasmid

Beta-actin is a highly conserved protein ubiquitously expressed in all eukaryotic cells as a component of the cytoskeleton and as a mediator of internal cell motility. A 1.2-kb fragment of the β-actin 5’ flanking region is sufficient for efficient transcription [1].

This 1.2-kb fragment contains a TATA box, a CCAAT box, and two highly conserved elements. The region between +765 and +783 downstream from the cap site has enhancer activity and acts as a serum-response element in quiescent cells [2].

This 19-bp element contains a CC(A/T)6GG sequence which is found in the promoter region of many cytoskeletal and muscle-specific actin genes.

The human β-actin promoter was shown to drive high levels of expression of a transgene in vitro [3]. InvivoGen provides a larger fragment that includes the  5’UTR of the β-actin gene.

References:

1. Sugiyama H. et al. 1988. Strong transcriptional promoter in the 5' upstream region of the human beta-actin gene. Gene. 65(1):135-9. 2. Orita S. et al., 1989. Identification of a site that mediates transcriptional response of the human beta-actin gene to serum factors. Gene.75(1):13-9.
3. Muller SR. et al. 1990. Efficient transfection and expression of heterologous genes in PC12 cells. DNA Cell Biol 9(3):221-9.

Specifications

pDRIVE bearing human β-Act promoter - LacZ, SEAP or Lucia

Gene Promoter: Beta-Actin

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin™ (100 mg/ml)

Product is shipped at room temperature

Details