pDRIVE-AFP

AFP promoter (Hepatocellular carcinoma) in pDRIVE expression plasmid

ABOUT

AFP promoter (Hepatocellular carcinoma) in pDRIVE expression plasmid

The alpha-fetoprotein (AFP) gene is normally expressed in fetal but not adult livers. However, about 70% of hepatocellular carcinoma (HCC) are known to overexpress AFP, this up-regulation occuring at the transcriptional level.

The AFP promoter has been extensively studied and shown to confer a selective expression of a transgene in vitro and in vivo [1].

The expression level of the transgene is proportional to the level of AFP expression in the transfected cells [2].

Several studies have reported the use of AFP promoter to express a cytokine or suicide gene into HCC cells after delivery with viral or plasmid vectors [1, 2]. 

 

References:

1. Kanai F. et al. 1997. In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene. Cancer Res. 57(3):461-5.
2. He P. et al. 2000. The targeted expression of interleukin-2 in human hepatocellular carcinoma cells. J Exp Clin Cancer Res. 19(2):183-7.

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

in vitro or in vivo transfection

Species
Human
Plasmid backbone
pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Antibiotic resistance
Zeocin®
Gene promoter
Alpha-Fetoprotein (AFP)
Specificity

Hepatocellular carcinoma

Reporter gene
Lac Z
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing.

CONTENTS

Contents

  • Product: 
    pDRIVE-hAFP
  • Cat code: 
    pdrive-hafp
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pDRIVE-hAFP

Technical Data Sheet

Plasmid Map and Sequence

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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