pBOOST4-mFLT3L-mGMCSF

Genetic adjuvant for DNA vaccines featuring the murine mFLT3L and GM-CSF genes

pBOOST4-mFLT3L-mGMCSF plasmid encodes the murine Fms-like tyrosine kinase 3 ligand (FLT3L) and granulocyte-macrophage colony-stimulating factor (GM-CSF).

FLTL3L is a synergistic hematopoietic growth factor that mediates cell survival, cell proliferation, and differentiation of dendritic cells [1]. GM-CSF, also known as colony-stimulating factor 2 (CSF2), is an important hematopoietic growth factor and immune modulator [2].  Mouse co-immunization with both FLT3L and GM-CSF encoding plasmids has been reported to increase antibody titer up to 9-fold [3].

Murine FLT3L and GMCSF genes are under the control of two strong composite promoters derived from the ferritin heavy chain (hFerH) and light chain (hFerL) core promoters, respectively.

pBOOST4-mFLT3L-mGMCSF plasmid is selectable in E. coli with Blasticidin.

Note: Murine FLT3L and GMCSF genes are also available in two separate plasmids, pUNO1-mFLT3LGa and pUNO1-mGMCSF.

References:

1. Shao Z. et al., 2009. Fms-like tyrosine kinase 3 ligand regulates migratory pattern and antigen uptake of lung dendritic cell subsets in a murine model of allergic airway inflammation. J Immunol. 183(11):7531-8.
2. Shi Y. et al., 2006. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and T-cell responses: what we do and don’t know. Cell Res. 16(2):126-33.
3. Chambers R.S. & Johnston S.A., 2003. High-level generation of polyclonal antibodies by genetic immunization. Nat Biotechnol. 21(9):1088-92.