MPLA Synthetic

TLR4 Agonist - Synthetic monophosphoryl lipid A

Synthetic lipid A from E. coli (MPLAs) is a monophosphoryl lipid A with 6 fatty acyl groups. It is structurally very similar to natural MPLA except that natural MPLA contains a mixture of 5, 6, and 7 acyl lipid A. The number of fatty acids is a major determinant of the immunogenicity of endotoxin.

Synthetic E. coli lipid A activates TLR4 but does not activate TLR2 even at high concentrations reflecting its high purity.

Specifications

CAS Number: 1246298-63-4

Formula: C₉₆H₁₈₄N₃O₂₂P

Molecular weight: 1763.47

Endotoxin level: 1 x 10e6 EU/mg

Solubility: 1 mg/ml in DMSO

Contents

MPLAs is provided lyophilized.

• 1 mg Synthetic Monophosphoryl Lipid A (MPLAs)  

MPLAs is shipped at room temperature.

Store at -20°C. Upon resuspension, prepare aliquots of MPLAs and store at -20°C.

Resuspended product is stable 6 months at -20°C. 

Avoid repeated freeze-thaw cycles.

Description

Synthetic lipid A from E. coli (MPLAs) is a monophosphoryl lipid A with 6 fatty acyl groups. It is structurally very similar to natural MPLA except that natural MPLA contains a mixture of 5, 6, and 7 acyl lipid A. The number of fatty acids is a major determinant of the immunogenicity of endotoxin. The most active form of lipid A contains six fatty acyl groups and is found in pathogenic bacteria such as E. coli and Salmonella species. MPLA, used extensively as a vaccine adjuvant, has been shown to activate TLR4 [1, 2]. Synthetic E. coli lipid A activates TLR4 but does not activate TLR2 even at high concentrations reflecting its high purity.

1. Martin M. et al., 2003. Role of innate immune factors in the adjuvant activity of monophosphoryl lipid A. Infect Immun. 71(5):2498-507.
2. Ogawa T. et al., 2002. Cell activation by Porphyromonas gingivalis lipid A molecule through Toll-like receptor 4- and myeloid differentiation factor 88-dependent signaling pathway. Int Immunol. 14(11):1325-32.