Anti-VISTA (Biosimilar antibody - IgG1fut isotype)
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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Anti-hVISTA-hIgG1fut Human VISTA biosimilar antibody - human IgG1, non-fucosylated |
Show product |
100 µg 3 x 100 µg |
hvista-mab13
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Non-fucosylated human IgG1 monoclonal antibody (mAb) against human VISTA
VISTA in the context of immunotherapy
Anti-hVISTA-hIgG1fut is a recombinant monoclonal antibody (mAb) featuring a variable region that recognizes human VISTA, a member of the CD28/B7 protein superfamily, and a non-fucosylated constant region of the human IgG1 (hIgG1fut) isotype.
V-domain Ig-containing suppressor of T-cell activation (VISTA), also known as programmed death-1 homolog (PH-1H) is a known immune checkpoint, that directly suppresses T cell activation, proliferation, and cytokine production [1]. VISTA can be considered both a ligand and a receptor, whereby it transmits inhibitory signals to T cells, either extrinsically, through expression on myeloid cells (i.e. macrophage and dendritic cells) and binding to an unknown receptor on T cells, or intrinsically, through its expression on T cells (i.e. CD4+, CD8+) [2]. In the context of cancer immunotherapy, VISTA has been found to be heightened on cells that infiltrate the tumor microenvironment (TME) and not the tumor cells themselves, therefore, VISTA-blocking antibodies are of interest. These function to inhibit the engagement of VISTA with its receptor on T cells or by directly targeting VISTA expressed on naïve and effector T cells, preventing it from providing negative signaling [1, 2].
Features of Anti-hVISTA-hIgG1fut:
- Targets specifically human VISTA
- Features the hIgG1fut constant region for enhanced effector function
- Functionally validated by flow cytometry
Anti-hVISTA-hIgG1fut is a non-fucosylated antibody, whereby the absence of fucose residues from N-glycans of the IgG-Fc results in an enhancement of antibody-dependent cellular cytotoxicity (ADCC). This increased ADCC is without any detectable change to complement-dependent cytotoxicity (CDC) or antigen-binding capability. Anti-hVISTA-hIgG1fut was generated by recombinant DNA technology, produced in CHO cells (deficient for fucosylation), and purified by affinity chromatography with protein G.
References:
1. Nowak, E.C. et al. 2017. Immunoregulatory functions of VISTA. Immunol Rev 276, 66-79.
2. Xu, W. et al. 2018. The structure, expression, and multifaceted role of immune-checkpoint protein VISTA as a critical regulator of anti-tumor immunity, autoimmunity, and inflammation. Cell Mol Immunol 15, 438-446.
Specifications
Specificity: Targets cells expressing human VISTA (V-domain Ig containing suppressor of T-cell activation)
Clonality: Monoclonal antibody
Isotype: Human IgG1fut, kappa
Control: Human IgG1fut
Source: CHO cells
Formulation: 0.2 μm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents.
Purification: Purified by affinity chromatography with protein G
Applications: Anti-hVSITA-hIgG1fut binds and blocks ligand activation of the VISTA receptor found on the surface of cells
Quality control:
- Binding of Anti-hVISTA-hIgG1fut to human VISTA on cells has been validated using flow cytometry.
- The complete sequence of the antibody has been verified.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cellular assays.
Contents
Anti-hVISTA-hIgG1fut purified monoclonal antibody is provided azide-free and lyophilized. It is available in two quantities:
- hvista-mab13: 100 µg
- hvista-mab13-03: 3 x 100 µg
Product is shipped at room temperature.
Upon receipt, store at -20 °C.
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