Human CD20 Antibody - Rituximab Mouse IgG1 Isotype

Mouse IgG1

ABOUT

Murine IgG1 monoclonal antibody (mAb) against human CD20

Anti-hCD20-mIgG1 features the constant region of the mouse IgG1 isotype and the variable region of rituximab.

Rituximab is a mouse/human chimeric monoclonal antibody that targets the CD20 antigen found on the surface of malignant and normal B lymphocytes.

The binding of rituximab to CD20 results in cell destruction through different mechanisms including direct signaling of apoptosis, complement activation, and cell-mediated cytotoxicity.

Rituximab has been approved by the FDA to treat various lymphoid malignancies, including B-cell non-Hodgkin's lymphoma and B-cell chronic lymphocytic leukemia.

Anti-hCD20-mIgG1 was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography with protein G.

More isotypes of this antibody are available and can be used for comparison of biological activities such as ADCC (see below or in the 'upon request' section). InvivoGen also provides type I and type II anti-hCD20 mAbs featuring the variable regions of Ofatumumab or Obinutuzumab, with either native or engineered Fc regions.

All products are for research use only, and not for human or veterinary use.

Effector functions of mAb isotypes targeting CD20
Effector functions of mAb isotypes targeting CD20

SPECIFICATIONS

Specifications

Target

CD20

Target species

Human

Applications

Flow cytometry, ADCC

Isotype
mIgG1
kappa
Recommended isotype control
Anti-β-Gal-mIgG1
Clone
Rituximab (Anti-hCD20-hIgG1, kappa)
Source
CHO cells
Production details
Animal-free
Purification
Protein G
Formulation buffer

0.2 µm filtered solution in 68 mM sodium phosphate buffer (pH 7.4) with 91 mM glycine, 5% w/v saccharose, and stabilizing agents

Preservative
Azide-free
Reconstitution buffer
Sterile water
Sterility

0.2 µm filtration

Tested applications

Flow cytometry, ADCC

Quality control

Binding of Anti-hCD20-hIgG1 to human CD20 tested using flow cytometry, Complete sequence verified, Absence of bacterial contamination (e.g. lipoproteins and endotoxins) confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells

CONTENTS

Contents

  • Product: 
    Anti-hCD20-mIgG1
  • Cat code: 
    hcd20-mab9
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: Stable for 1 month when stored at 4°C and for 1 year when aliquoted and stored at -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-hCD20-mIgG1

Technical Data Sheet

Safety Data Sheet

View document

Certificate of analysis

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Citations

CUSTOMER SERVICE & TECHNICAL SUPPORT

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