Human CD20 Antibody - Obinutuzumab Biosimilar

Human IgG1, afucosylated - CAS #949142-50-1 

ABOUT

Non-fucosylated human IgG1 monoclonal antibody (mAb) against human CD20

Anti-hCD20-Ga-hIgG1fut is the biosimilar of the clinical antibody obinutuzumab. Anti-hCD20-Ga-hIgG1fut features the constant region (Fc) of a non-fucosylated (fut), human IgG1 isotype and the variable region of obinutuzumab. Obinutuzumab (formerly named afutuzumab or GA101) targets the human CD20 antigen found on the surface of normal and malignant B lymphocytes.

Obinutuzumab is a type II (second generation) non-fucosylated, fully humanized IgG1 monoclonal antibody with improved therapeutic efficacy compared with previously developed type I (first generation) anti-hCD20 mAbs, such as Rituximab and Ofatumumab. Obinutuzumab has been approved by the FDA to treat chronic lymphocytic leukemia [1-3].

The absence of fucose residues from the Fc N-glycans results in dramatic enhancement of antibody-dependent cellular cytotoxicity (ADCC) without any detectable change in complement-dependent cytotoxicity (CDC) or antigen-binding capability [4].

 

InvivoGen’s Anti-hCD20-Ga-hIgG1fut is generated by recombinant DNA technology, produced in Chinese hamster ovary (CHO) cells deficient for fucosylation, and purified by affinity chromatography (protein G).

More isotypes of this antibody are available and can be used for comparison of biological activities such as ADCC (see below or in the ' upon request' section).
InvivoGen also provides type I anti-hCD20 mAbs featuring the variable regions of Rituximab or Ofatumumab, with either native or engineered Fc region.

 

Read our review on Antibody Isotypes

 

References:

1. Gagez AL. & Cartron G., 2014. Obinutuzumab: a new class of anti-CD20 monoclonal antibody. Curr Opin Oncol. 26(5):484-91.
2. Smolej L., 2014. Targeted treatment for chronic lymphocytic leukemia: clinical potential of obinutuzumab. Pharmgenomics Pers Med. 8:1-7.
3. Winiarska M. et al., 2011. Molecular mechanisms of the antitumor effects of anti-CD20 antibodies. Front Biosci (Landmark Ed). 16:277-306. 4. Mizushima T., 2011. Structural basis for improved efficacy of therapeutic antibodies on defucosylation of their Fc glycans. Genes Cells. 16(11): 1071–1080.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

CD20

Target species

Human

Applications

Flow cytometry

Isotype
hIgG1fut
kappa
Clone
Obinutuzumab (Anti-hCD20-Ga-hIgG1fut, kappa)
Source
CHO cells
Purification
Protein G
Formulation buffer

68 mM sodium phosphate buffer (pH 7.4) with 91 mM glycine, 5% w/v saccharose, and stabilizing agents

Preservative
Azide-free
Reconstitution buffer
Sterile water
Sterility

0.2 µm filtered

Tested applications

Flow cytometry

Quality control

Binding of Anti-hCD20-Ga-hIgG1fut to human CD20 tested using flow cytometry, Complete sequence verified, Absence of bacterial contamination (e.g. lipoproteins and endotoxins) confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells

CONTENTS

Contents

  • Product: 
    Anti-hCD20-Ga-hIgG1fut
  • Cat code: 
    hcd20ga-mab13
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Stable for 1 month when stored at 4°C and for 1 year when aliquoted and stored at -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-hCD20-Ga-hIgG1fut

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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