Anti-CD20 (Rituximab biosimilar - IgG3 isotype)

Human IgG3 monoclonal antibody (mAb) against human CD20

Anti-hCD20-hIgG3 features the constant region of the human IgG3 isotype and the variable region of rituximab.

Rituximab is a mouse/human chimeric monoclonal antibody that targets the CD20 antigen found on the surface of malignant and normal B lymphocytes.

The binding of rituximab to CD20 results in cell destruction through different mechanisms including direct signaling of apoptosis, complement activation, and cell-mediated cytotoxicity.

Rituximab has been approved by the FDA to treat various lymphoid malignancies, including B-cell non-Hodgkin's lymphoma and B-cell chronic lymphocytic leukemia.

Anti-hCD20-hIgG3 was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography with protein G.

More isotypes of this antibody are available and can be used for comparison of biological activities such as ADCC (see below or in the 'upon request' section). InvivoGen also provides type I and type II anti-hCD20 mAbs featuring the variable regions of Ofatumumab or Obinutuzumab, with either native or engineered Fc regions.

Specifications

Target: Human CD20

Clonality: Monoclonal antibody

Clone: Rituximab (Anti-hCD20-hIgG1, kappa)

Isotype: Human IgG3, kappa

Control: Human IgG3

Source: CHO cells

Formulation: 0.2 µm filtered solution in 68 mM sodium phosphate buffer (pH 7.4) with 91 mM glycine, 5% w/v saccharose, and stabilizing agents

Purity: Purified by affinity chromatography with protein G

Tested application: Flow cytometry and ADCC

Quality control:

• Binding of Anti-hCD20-hIgG3 to human CD20 has been tested using flow cytometry.
• The complete sequence of this antibody has been verified.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

Anti-hCD20-hIgG3 purified monoclonal antibody is provided azide-free and lyophilized. It is available in two quantities:

• hcd20-mab3: 100 µg
• hcd20-mab3-03: 3 x 100 µg

Product is shipped at room temperature.

 Upon receipt, store lyophilized antibody at -20°C.