Anti-hCD20-Of-hIgA2

Monoclonal human IgA2 antibody against human CD20

Anti-hCD20-Of-hIgA2 features a human IgA2 constant region (Fc) and the variable region of Ofatumumab which targets the human CD20 antigen found on the surface of normal and malignant B lymphocytes.

Ofatumumab is a fully human type I (first generation) monoclonal antibody which mediates B cell destruction through complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC). Ofatumumab has been approved by the FDA for the treatment of chronic lymphocytic leukaemia (CLL)  [1-3].

InvivoGen’s Anti-hCD20-Of-hIgA2 is generated by recombinant DNA technology, produced in CHO cells, and purified by affinity chromatography (peptide M).
More isotypes of this antibody are available and can be used for comparison of biological activities such as ADCC (see below).
InvivoGen also provides type I anti-hCD20 mAbs featuring the variable regions of Rituximab or Obinutuzumab, with either native or engineered Fc region.

 Read our review on Antibody Isotypes

References:

1. Baig NA. et al., 2014. Induced resistance to ofatumumab-mediated cell clearance mechanisms, including complement-dependent cytotoxicity, in chronic lymphocytic leukemia. J Immunol. 192(4):1620-9.
2. Lin TS., 2010. Ofatumumab: a novel monoclonal anti-CD20 antibody. Pharmgenomics Pers Med. 3:51-9.
3. Winiarska M. et al., 2011. Molecular mechanisms of the antitumor effects of anti-CD20 antibodies. Front Biosci (Landmark Ed). 16:277-306.

Specifications

Target: Human CD20
Clonality: Monoclonal antibody
Isotype: Human IgA2, kappa
Source: CHO cells
Formulation: 0.2 µm filtered solution 91 mM TRIS buffer with 91 mM glycine, 5% w/v saccharose and stabilizing agents
Purity: Purified by affinity chromatography with peptide M

Tested application: Flow cytometry

Quality control:

• Binding of Anti-hCD20-Of-hIgA2 to human CD20 has been tested using flow cytometry.
• The complete sequence of this antibody has been verified.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

• 100 µg purified Anti-hCD20-Of-hIgA2 monoclonal antibody (mAb), provided azide-free and lyophilized

Anti-hCD20-Of-hIgA2 is shipped at room temperature.

Store lyophilized antibody at -20 °C.