Control Raji Cells

Human lymphoblast cells - ADCC CD19/CD20/Control Target Cells

ABOUT

Control Human B-cell line

Raji-Null cells were developed from the human Raji cell line. Raji-Null cells constitutively express different antigens at their cell surface that can be targeted by specific antibodies, notably to induce antibody-dependent cellular cytotoxicity (ADCC).

-Human CD20 is highly expressed by Raji cells. It has no known natural ligand and is thought to act as calcium channel enabling optimal B-cell responses [1]. Human CD20 is a clinically-relevant target in mAb-mediated therapy (rituximab, ofatumumab, obinutuzumab) for non-Hodgkin's B cell lymphoma or chronic lymphocytic leukemia [2].

-Human CD19 is highly expressed by Raji cells. It acts as a co-receptor decreasing the threshold for antigen B-cell receptor (BCR)-dependent stimulation [1]. Human CD19 is a clinical target for anti-hCD19-CD3 bis-specific antibodies (blinatumomab) in non Hodgkin's B cell lymphoma [3].

- Human PD-L1 (programmed cell death ligand 1; also known as CD274 or B7-H1) is expressed at low levels by Raji cells. This transmembrane protein is expressed by hematopoietic and nonhematopoietic cells and is induced by pro-inflammatory cytokines, such as in the tumor microenvironment [4]. Human PD-L1 is a clinically-relevant target in mAb-mediated therapy (atezolizumab, durvalumab, avelumab) for a wide range of cancers [4].

Features of Raji-Null cells:

  • Constitutive expression of CD19 and CD20 at the cell surface
  • Constitutive low expression of PD-L1 at the cell surface
  • Stable expression of a resistance gene to Blasticidin

Applications for Raji-Null cells:

  • ADCC target cells using anti-hCD19, anti-hCD20, anti-hPD-L1 Abs
  • Negative control target cells in ADCC assays using monoclonal Abs other than anti-hCD19, anti-hCD20, anti-hPD-L1 Abs
  • Target cells for anti-hCD19-CD3 bispecific Abs

Validation of Raji-Null cells:

 

References:

1. Bae J. et al. 2005. Identification of CD19 and CD20 peptides for the induction of antigen-specific CTLs against B-cell malignancies. Clin. Cancer. Res. 11:1629-38.
2. Almagro J.C. et al. 2018. Progress and challenges in the development of antibodies for cancer therapy. Front. Immunol. 8:1751.
3. Bargou R. et al. 2008. Tumor regression in cancer patients by very low doses of a T cell-engaging antibody. Science. 321(5891):974-7.
4. Ribas A. and Wolchock J.D., 2018. Cancer immunotherapy using checkpoint blockade. Science. 359:1350-55.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Human
Target

CD19, CD20, PD-L1

Target species

Human

Tested applications

ADCC induction cellular assays,  T-cell activation cellular assays, Flow cytometry

Cell type
Lymphoblastic
Growth properties
Suspension
Tissue origin
Human B cell lymphoma
Antibiotic resistance
Blasticidin
Mycoplasma-free

Verified using Plasmotest™

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Raji-Null Cells
  • Cat code: 
    raji-null
  • Quantity: 
    3-7 x 10^6 cells
Includes:
  • 1 ml of Blasticidin (10 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

DOCUMENTS

Documents

Raji-Null Cells

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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