Human PD-1-expressing Raji Cells
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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Raji-hPD-1 Cells Human lymphoblast cells - ADCC PD-1 Target Cells |
Show product |
3-7 x 10e6 cells |
raji-hpd1
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Human PD-1-expressing B cells
Raji-hPD-1 cells were developed from the Raji cell line, a human B lymphocyte-derived cell line. Raji cells have been successfully used as target cells in CAR-T toxicity assays as well as human effector studies such as antibody-dependent cellular cytotoxicity (ADCC), either with peripheral blood mononuclear cells, natural killer (NK) cells, or T cell-derived Jurkat reporter cells.
PD-1 (programmed cell death 1; also known as CD279) is a type I transmembrane protein expressed at the cell surface of activated and exhausted conventional T cells. PD-1 is an inhibitory immune checkpoint that prevents T-cell overstimulation and host damage. PD-1 interaction with its ligands PD-L1 and PD-L2 induces inhibition of T-cell receptor signaling [1].
Features of Raji-hPD-1 cells:
Surface expressed markers and ICs in Raji-hPD-1 cells
- Stable overexpression of the human PD-1 gene
- Characterized by a number of cell-surface expressed markers including the B cell receptor (BCR), CD19, and CD20
- Constitutive expression of various immune checkpoints (ICs) such as CD27, CD70, CD80, PD-L1, and 4-1BBL
- The stability for 20 passages following thawing has been verified
Applications for Raji-hPD-1 cells:
- Target cell line for ADCC assays using InvivoGen's Jurkat-Lucia™ NFAT-CD16 cells
- Target cell line for cell toxicity assays using NK or CAR-T cells
- For use in the development of other functional assays
Validation of Raji-hPD-1 cells:
- Overexpression of PD-1 verified by flow cytometry
- Functionally tested as target cells in ADCC assays using anti-human PD-1 monoclonal Abs and Jurkat-Lucia™ NFAT-CD16 cells
- Guaranteed mycoplasma-free
References:
1. Ribas A. and Wolchock J.D., 2018. Cancer immunotherapy using checkpoint blockade. Science. 359:1350-55.
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Antibiotic resistance: Blasticidin
Growth medium: IMDM, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (FBS; 30 min at 56 °C), 10 μg/ml Blasticidin, Pen-Strep (100 U/ml-100 µg/ml)
Test medium: IMDM, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated FBS, Pen-Strep (100 U/ml-100 µg/ml)
Quality control:
- Human PD-1 expression has been verified by flow cytometry.
- Induction of antibody-dependent cellular cytotoxicity (ADCC) has been validated using InvivoGen’s anti-hPD-1-hIgG1 antibody and Jurkat-NFAT Lucia™ CD16 reporter cell line.
- The stability for 20 passages following thawing has been verified.
- Raji-hPD-1 cells are guaranteed mycoplasma-free.
Contents
- 3-7 x 106 Raji-hPD-1 cells in a cryovial or shipping flask.
- 1 ml of Blasticidin (10 mg/ml). Store at 4 °C or at -20 °C.
- 1 ml of Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. Store at -20 °C.
IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.
Shipped on dry ice (Europe, USA & Canada)
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