Anti-β-Gal-hIgG3

Monoclonal human IgG3 antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG3 features the constant region of the human IgG3 isotype and the variable region of Mouse IgG2a Control. Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal). This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice. It can be used as an isotype control for human IgG3 antibodies.
Human IgG3 is the third most common antibody present in serum. Human IgG3 is very susceptible to cleavage by proteolytic enzymes, due an extended hinge region. Human IgG3 displays strong antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC).
Anti-β-Gal-hIgG3 was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity  chromatography.

Specifications

Clonality: Monoclonal antibody

Specificity: Targets cells expressing E. coli β-galactosidase (β-Gal)

Isotype: Human IgG3, kappa

Application: Negative control for human IgG3 antibodies

Quality control:

• The absence of binding of Anti-β-Gal-hIgG3 to human cell lines has been controlled using flow cytometry.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

Contents

• 200 µg purified anti-β-Gal-IgG3 antibody, provided azide-free and lyophilized

Product is shipped at room temperature.

Store lyophilized antibody at -20 °C.

Lyophilized product is stable for at least 1 year