Human IgG3 Isotype Control Antibody

Anti-β-galactosidase antibody - Human IgG3 - Recombinant

ABOUT

Recombinant human IgG3 control mAb against E. coli β-galactosidase

Anti-β-Gal-hIgG3 features the constant region of the human IgG3 isotype and the variable region of Mouse IgG2a Control. Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal), an antigen that is not present in humans or mice. This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice. It can be used as an isotype control for human IgG3 antibodies.

Human IgG3 is the third most common antibody present in serum. Human IgG3 is very susceptible to cleavage by proteolytic enzymes, due to an extended hinge region. Human IgG3 displays strong antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC).

Anti-β-Gal-hIgG3 was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

β-Galactosidase

Target species

E. coli

Applications

Negative control antibody

Isotype
hIgG3
kappa
Clone
T9C6
Tag
Tag-free
Source
CHO cells
Purification
Affinity chromatography
Formulation buffer

Sodium phosphate buffer, glycine, saccharose, stabilizing agents

Preservative
Azide-free
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Negative control for cellular assays, flow cytometry, ELISA

Quality control

Each lot is validated by flow cytometry.

CONTENTS

Contents

  • Product: 
    Anti-β-Gal-hIgG3
  • Cat code: 
    bgal-mab3
  • Quantity: 
    Custom size

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20 °C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-β-Gal-hIgG3

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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