Recombinant Mouse IgG2a Isotype Control Antibody - Fc silent (LALA-PG)

Recombinant effectorless (LALA-PG) mouse IgG2a isotype control mAb against E. coli β-galactosidase

The recombinant Anti-β-Gal-mIgG2ae3 is an Fc silent monoclonal antibody (mAb) that serve as a negative control antibody in vivo. 

This control antibody targets E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. Its engineered mouse IgG2a isotype contains three point mutations in the Fc region at positions L234A, L235A, and P329G. These mutations, also referred to as LALA-PG, result in the complete loss of unwanted Fc-associated effector functions.

Anti-β-Gal-mIgG2ae3 is expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. Due to the triple mutation LALA-PG, it displays no ADCP (antibody-dependent cell-mediated phagocytosis), no ADCC (antibody-dependent cell-mediated cytotoxicity) and no CDC (complement-dependent cytotoxicity) effector functions. 

The sequence of Anti-β-Gal-mIgG2ae3 InvivoFit™ is 100% murine (constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].

Key features:

• Sequence is 100% murine
• Engineered IgG2a isotype (IgG2ae3)
• No effector functions
• Free from non-relevant mAbs found in hybridoma-based productions
• Produced in animal-free facilities and defined media
• Low aggregation < 5%
• InvivoFit™ grade

This antibody is also available in bulk quantity, please contact us