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QUANTI-Luc™ Gold [ NEW ]

QUANTI-Luc™ Gold Unit size Cat. code Docs Price
Coelenterazine-based luminescence detection kit for standard and HTS assays
1 pouch (25 ml)
2 pouches (2 x 25 ml)
5 pouches (5 x 25 ml)
1 pouch (500 ml)
rep-qlcg1
$134.00

NEW PRODUCT

QUANTI-Luc™ Gold | InvivoGen

Coelenterazine-based luminescence detection kit for standard and HTS assays

QUANTI-Luc™ Gold is an optimized kit for the detection of Lucia luciferase and other coelenterazine-utilizing luciferases (e.g. Gaussia and Renilla luciferases).

The light signal produced by the luciferase reaction with the coelenterazine substrate is quantified using a luminometer and expressed as relative light units (RLU).

 

This two-component kit comprises:

  • QUANTI-Luc™ Plus, a coelenterazine-containing reagent
  • QLC Stabilizer

This kit provides the user with two options for luciferase activity measurement:

  • QUANTI-Luc™ Gold (QUANTI-Luc™ Plus with QLC Stabilizer) allows enhanced light signal stability. This option is ideal for high-throughput screening (HTS), time-course studies, or when using a luminometer without injectors.
  • QUANTI-Luc™ Plus alone (without QLC Stabilizer) allows enhanced light output detection (as compared to QUANTI-Luc™). This option is ideal for monitoring the cellular response to weak agonists.

 

 

Enhanced light signal stability with QUANTI-Luc™ Gold
Enhanced light signal stability with QUANTI-Luc™ Gold

Lucia luciferase activity detection with QUANTI-Luc™ Gold (QUANTI-Luc™ Plus with QLC Stabilizer), QUANTI-Luc™ Plus (without QLC Stabilizer) and QUANTI-Luc™. THP1‑Dual™ cells were treated with a STING agonist, 2’3’-cGAMP (10 μg/ml), to activate the interferon regulatory factor (IRF)-inducible Lucia luciferase reporter gene. After overnight incubation, the IRF response was determined by measuring the relative light units (RLUs) with a luminometer using 20 μl of cell culture supernatant and 50 μl of detection medium. RLUs were measured every minute for 10 minutes.

Stabilization of light signal with QLC Stabilizer
Stabilization of light signal with QLC Stabilizer

Monitoring of Lucia luciferase reporter activity using QUANTI-Luc™ Plus alone or with QLC Stabilizer (QUANTI-Luc™ Gold) upon variation of agonist concentration. THP1-Dual™ cells were treated with increasing concentrations of 2’3’-cGAMP to activate the IRF-inducible Lucia luciferase reporter gene. After overnight incubation, the IRF response was determined by measuring the relative light units (RLUs) with a luminometer using 20 μl of cell culture supernatant and 50 μl of detection medium. RLUs were measured every minute for 10 minutes. The dose-response curves for QUANTI-Luc™ Gold superimpose for each time measurement, which demonstrate the stability of the light signal.

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Specifications

Flexible: Two options: enhanced light signal stability or enhanced light output

Cost effective: One pouch prepares 5 x 96-well plates or 2 x 1536-well plates

Practical: Reconstituted QUANTI-Luc™ Gold is stable for 1 week at 4°C or 1 month at -20°C

Exceptional sensitivity and reproducibility

Quality control: Each lot is thoroughly tested in functional assays using the recombinant Lucia protein or Lucia reporter cells.

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Contents

QUANTI-Luc™ Gold is available in four pack sizes:

  • rep-qlcg1: 1 pouch of QUANTI-Luc™ Plus and 1 tube of QLC Stabilizer
  • rep-qlcg2: 2 pouches of QUANTI-Luc™ Plus and 2 tubes of QLC Stabilizer
  • rep-qlcg 5: 5 pouches of QUANTI-Luc™ Plus and 5 tubes of QLC Stabilizer
  • rep-qlcg500: 1 pouch of QUANTI-Luc™ XPlus and 14 tubes of QLC Stabilizer

One pouch of QUANTI-Luc™ Plus provides sufficient reagent for 5 x 96-well plates (standard procedure) or 2 x 1536-well plates (HTS procedure).

One pouch of QUANTI-Luc™ XPlus provides sufficient reagent for 100 x 96-well plates (standard procedure) or 40 x 1536-well plates (HTS procedure).

QUANTI-Luc™ Plus and QLC Stabilizer are shipped at room temperature.

Upon receipt, store QUANTI-Luc™ Plus or QUANTI-Luc™ XPlus at -20°C, and QLC Stabilizer at room temperature (15-25°C).

Note: This product is photosensitive and should be protected from light.

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