RIG-I
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pUNO1-hRIGI pUNO1 bearing the human RIG-I gene |
Show product |
20 µg |
puno1-hrigi
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pUNO1-hRIGI-DN pUNO1 bearing a dominant negative version of the human RIG-I gene |
Show product |
20 µg |
puno1-hrigi-dn
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pUNO1-mRIGI pUNO1 bearing the mouse RIG-I gene |
Show product |
20 µg |
puno1-mrigi
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Expression-ready ORF Clones
Alias : DDX58, RIG-I
RNA helicase retinoic acid inducible gene I.
DEAD [Asp-Glu-Ala-Asp] box polypeptide 58.
Available versions:
- Wild type RIG-I
-
RIG-I DN: Dominant negative RIG-I (also called RIG-IC)
Dominant negative RIG-I (RIG-IC) was generated by removing the two caspase recruitment domains (CARD) found at the N terminus (aa1-217). Expression of RIG-IC in L929 cells was shown to inhibit virus-induced gene expression [1].
References
1. Yoneyama M. et al., 2005. Shared and Unique Functions of the DExD/H-Box Helicases RIG-I, MDA5, and LGP2 in Antiviral Innate Immunity. J. Immunol. 175:2851-58.
Back to the topSpecifications
Human RIG-I (pUNO1-hRIGI)
Genbank : NM_014314.3
ORF size : 2778 bp
Subclone : AgeI - NheI
Human RIG-I-DN (pUNO1-hRIGI-DN)
ORF size : 2130 bp
Subclone : AgeI - NheI
Mouse RIG-I (pUNO1-mRIGI)
ORF size : 2781 bp
Subclone : BspHI - XbaI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.