pUNO1-hPYCARD-DN
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Cat.code:
puno1-hpycard-dn
- Documents
Expression-ready ORF Clones
Alias: ASC, CARD5, TMS1
PYD and CARD domain containing
Apoptosis-associated speck-like protein containing a CARD
Dominant negative PYCARD
The dominant negative version of PYCARD (ASC) is a truncated form of the gene (aa1-94) that contains the Pyrin domain but not the CARD domain. PYCARD-DN binds to NALP1 but not caspase-1 and strongly blocks the maturation of IL-1β in PMA-differentiated THP1 cells stimulated with LPS without affecting LPS-induced proIL-1β synthesis [1]
1. Martinon F. et al., 2002. The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-1β. Mol Cell (10): 417-426.
Specifications
Human PYCARD-DN (pUNO1-hPYCARD-DN)
ORF size : 285 bp
Subclone : AgeI - NheI
Mouse PYCARD-DN (pUNO1-mPYCARD-DN)
ORF size : 282 bp
Subclone : NcoI - NheI
Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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