PYCARD - ASC
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pUNO1-hPYCARDa pUNO1 bearing the human PYCARD (ASC) gene, isoform 1 (long) |
Show product |
20 µg |
puno1-hpycarda
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pUNO1-hPYCARD-DN pUNO1 bearing a dominant negative version of the human PYCARD (ASC) gene |
Show product |
20 µg |
puno1-hpycard-dn
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pUNO1-mPYCARD pUNO1 bearing the mouse PYCARD (ASC) gene |
Show product |
20 µg |
puno1-mpycard
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pUNO1-mPYCARD-DN pUNO1 bearing a dominant negative version of the mouse PYCARD (ASC) gene |
Show product |
20 µg |
puno1-mpycard-dn
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Expression-ready ORF Clones
Alias: ASC, CARD5, TMS1
PYD and CARD domain containing.
Apoptosis-associated speck-like protein containing a CARD.
Available versions:
- Wild type PYCARD
- Dominant negative PYCARD (see the "Details" tab)
Specifications
Human PYCARD isoform 1 (long) (pUNO1-hPYCARDa)
Genbank : NM_013258.4
ORF size : 588 bp
Subclone : AgeI - NheI
Human PYCARD-DN (pUNO1-hPYCARD-DN)
ORF size : 285 bp
Subclone : AgeI - NheI
Mouse PYCARD (pUNO1-mPYCARD)
Genbank : NM_023258.4
ORF size : 582 bp
Subclone : NcoI - NheI
Mouse PYCARD-DN (pUNO1-mPYCARD-DN)
ORF size : 282 bp
Subclone : NcoI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.
Details
PYCARD-DN version
The dominant negative version of PYCARD (ASC) is a truncated form of the gene (aa1-94) that contains the Pyrin domain but not the CARD domain. PYCARD-DN binds to NALP1 but not caspase-1 and strongly blocks maturation of IL-1β in PMA-differentiated THP1 cells stimulated with LPS without affecting LPS-induced proIL-1β synthesis [1].
1. Martinon F et al., 2002. The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-1β. Mol Cell (10): 417-426.
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