pUNO1-hPOU5F1a-11RHA3x
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Cat.code:
puno1rha-hpou5f1
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Expression-ready ORF Clones
Alias : OCT4, OTF3, OCT3
HA-tagged Octamer-binding expression factor 4 / POU domain class5 transcription factor 1,
with cell-penetrating peptide (11 Arg) at the C-terminus.
The gene has been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
Specifications
Human POU5F1a::11RHA (pUNO1-hPOU5F1a-11RHA3x)
ORF size : 1236 bp
Subclone : NcoI - NheI
Mouse POU5F1a::11RHA (pUNO1-mPOU5F1a-11RHA3x)
ORF size : 1473 bp
Subclone : NcoI - NheI
Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Plasmid Map and Sequence
Plasmid Sequence
Certificate of analysis
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