NOD1-DN
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
|
pUNO1-hNOD1-DN pUNO1 bearing a dominant negative version of the human NOD1 gene |
Show product |
20 µg |
puno1-hnod1-dn
|
|
Expression-ready ORF Clones
Alias : NLRC1, CARD4
Nucleotide-binding oligomerization domain containing 1
Caspase recruitment domain-containing protein 4
Dominant negative NOD1
Nucleotide-binding oligomerization domain 1 (NOD1), also called caspase-recruitment domain (CARD)-4, is an intracellular pathogen-recognition molecule expressed ubiquitously in adult tissues. Upon bacterial invasion, NOD1 induces NF-κB activation pathways [1]. NOD1 recognizes a naturally occurring muropeptide of peptidoglycan (PGN) that presents a unique amino acid at its terminus called diaminopilemic acid (DAP) [2].
This amino acid is found mainly in the PGN of gram-negative bacteria designating NOD1 as a sensor of gram-negative bacteria. Recent studies show that signaling through NOD1 is able to activate NF-κB in cells infected with gram-negative bacteria that can bypass TLR activation [3].
Dominant negative NOD1 was generated by a deletion of the centrally located nucleotide-binding site (NBS) domain (aa 127-518) [4].
References:
1. Inohara N. et al., 1999. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB. J Biol Chem. 274(21):14560-7.
2. Girardin SE. et al., 2003. Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science. 300(5625):1584-7.
3. Kim JG. et al., 2004. Nod1 is an essential signal transducer in intestinal epithelial cells infected with bacteria that avoid recognition by toll-like receptors. Infect Immun. 72(3):1487-95.
4. Bertin JJ et al. 1999. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem. 274(19):12955-8
Specifications
Human NOD1-DN (pUNO1-hNOD1-DN)
ORF size : 2490 bp
Subclone : NcoI - EcoRI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.