MyD88-DN

Expression-ready ORF Clones

Myeloid Differentiation Primary Response  88

Dominant negative MyD88 

Myeloid differentiation factor 88 (MyD88) is a cytoplasmic adaptor protein that is involved in interleukin-1 receptor (IL-1R)- and Toll-like receptor (TLR)-induced activation of NF-kappaB. MyD88 contains a C-terminal Toll/IL-1R homology (TIR) domain and an N-terminal death domain (DD), which mediate the interaction of MyD88 with IL-1R/TLR and IRAK, respectively [1]. MyD88 is essential for signaling as MyD88 -/- mice fail to induce cytokines in response to LPS and CpG DNA [2]. Some TLRs induce antiviral responses by producing IFN-alpha in a MyD88-dependent manner. MyD88 interacts with IRF7 resulting in the activation of IFN-alpha-dependent promoters [3].
 

1. Janssens S. et al., 2002. Regulation of interleukin-1- and lipopolysaccharide-induced NF-kappaB activation by alternative splicing of MyD88. Curr Biol. 12(6):467-71.
2. Kawai T. et al. 1999. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 11(1):115-22.
3. Kawai T. et al., 2004. Interferon-alpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6. Nat Immunol. 5(10):1061-8. 

Specifications

Human MyD88-DN (pUNO1-hMyD88-DN)

ORF size : 420 bp
Subclone : NcoI - NheI

Mouse MyD88-DN (pUNO1-mMyD88-DN)

ORF size : 420 bp
Subclone : NcoI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.