IRAK1-DN
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pUNO1-hIRAK1-DN pUNO1 bearing a dominant negative version of the human IRAK1 gene |
Show product |
20 µg |
puno1-hirak1-dn
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Expression-ready ORF Clones
Alias: IRAK, pelle
Interleukin-1 receptor-associated kinase 1
Dominant negative IRAK1
Interleukin-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that act as signal transduction mediators for the TLRs. The IRAK family consists of two active kinases, IRAK-1 and IRAK-4, and two inactive kinases, IRAK-2 and IRAK-M [1]. Upon stimulation, the adaptor MyD88 recruits IRAK-1, IRAK-2, and IRAK-4 to the receptor. IRAK-4 then phosphorylates IRAK-1 which dissociates from the receptor and interacts directly with TRAF-6 to relay the signal [2]. Subsequently, IRAK-1 undergoes autophosphorylation followed by degradation. IRAK-4 is an essential component of the IL-1, IL-18 and TLR signaling cascade [2-4].
Dominant negative IRAK1 (aa1-211) is a truncated form of IRAK1 that contains the N-terminal death domain but lacks the protein-kinase domain. This IRAK1-DN was shown to inhibit MyD88-induced NF-κB activation, suggesting that IRAK1 and MyD88 interact through their death domains [5].
References:
1. Janssens S. & Beyaert R. , 2003. Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members. Mol Cell. 11(2):293-302.
2. Li S. et al., 2002. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase.Proc Natl Acad Sci U S A. 99(8):5567-72.
3. Lye E. et al., 2004. The role of interleukin 1 receptor-associated kinase-4 (IRAK-4) kinase activity in IRAK-4-mediated signaling. J Biol Chem. 279(39):40653-8.
4. Suzuki N et al., 2003. IL-1 receptor-associated kinase 4 is essential for IL-18-mediated NK and Th1 cell responses. J Immunol. 170(8):4031-5.
5. Burns K. et al., 1998. MyD88, an adapter protein involved in interleukin-1 signaling. J Biol Chem. 273(20):12203-9.
Specifications
Human IRAK1-DN (pUNO1-hIRAK1-DN)
ORF size : 636 bp
Subclone : NcoI - NheI
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pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.