EIF2AK2
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pUNO1-hEIF2AK2 pUNO1 bearing the human EIF2AK2 (PKR) gene |
Show product |
20 µg |
puno1-heif2ak2
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pUNO1-hEIF2AK2-DN pUNO1 bearing a dominant negative version of the human EIF2AK2 (PKR) gene |
Show product |
20 µg |
puno1-heif2ak2-dn
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pUNO1-mEIF2AK2 pUNO1 bearing the mouse EIF2AK2 (PKR) gene |
Show product |
20 µg |
puno1-meif2ak2
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Expression-ready ORF Clones
Alias : PKR, PRKR, EIF2AK1
Eukaryotic translation initiation factor 2-alpha kinase 2.
Protein Kinase, Interferon-inducible double stranded RNA dependent.
Available versions:
- Wild type EIF2AK2
- Dominant negative EIF2AK2
Protein kinase RNA-regulated (PKR), also known as Eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2,) an ubiquitous serine/threonine kinase that is induced by interferons, is a critical signal transducer implicated in different stress-induced signaling pathways including dsRNA-induced TLR3 signaling [1] and LPS-induced TLR4 signaling [2,3]. Upon stress stimulation, PKR is activated by autophosphorylation and subsequently phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF-2a) thereby inducing apoptosis. PKR mediates the activation of important transcription factors, such as NF-kB, and MAP kinases, such as p382, by interacting with the TAK1 complex [4].
References
1. Jiang Z. et al., 2003. Poly(I-C)-induced Toll-like receptor 3 (TLR3)-mediated activation of NFkappa B and MAP kinase is through an interleukin-1 receptor-associated kinase (IRAK)-independent pathway employing the signaling components TLR3-TRAF6-TAK1-TAB2-PKR. J Biol Chem. 278(19):16713-9.
2. Goh KC. et al., 2000. The protein kinase PKR is required for p38 MAPK activation and the innate immune response to bacterial endotoxin. EMBO J. 19(16):4292-7.
3. Hsu LC. et al., 2004. The protein kinase PKR is required for macrophage apoptosis after activation of Toll-like receptor 4. Nature. 2004 Mar 18;428(6980):341-5.
Specifications
Human EIF2AK2 (pUNO1-hEIF2AK2)
Genbank : NM_002759.3
ORF size : 1656 bp
Subclone : AgeI - NheI
Human EIF2AK2-DN (pUNO1-hEIF2AK2-DN)
ORF size : 1638 bp
Subclone : AgeI - NheI
Mouse EIF2AK2 (pUNO1-mEIF2AK2)
ORF size : 1548 bp
Subclone : NcoI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.