codA::upp

Expression-ready ORF Clones

E. coli Cytosine Deaminase fused to Uracil Phosphoribosyltransferase.

Cytosine deaminase (CD) is an enzyme found in prokaryotes and lower eukaryotes but absent in higher eukaryotes. It converts cytosine and its analog, 5-fluorocytosine (5-FC), to uracil and 5-fluorouracil (5-FU) respectively. 5-FU is a toxic compound commonly used as an anticancer drug.
To further increase the efficiency of the CD/5-FC pair, InvivoGen has fused the gene encoding uracil phosphoribosyl transferase to the CD gene. UPRT converts 5-FU to 5-FUMP, an irreversible inhibitor of thymidylate synthase.