Protein L / Agarose

Agarose for IgA and IgG purification - k light chain specific

Protein L binds specifically to the variable domain of Ig κ light chain; as a consequence, Protein L has the capacity to purify κ light-containing IgA antibodies.

It should be noted that protein L binding is restricted to specific subclasses of the kappa domain.

As Protein L recognizes κ light chains, Protein L can bind to all classes of Ig, in contrast to Protein A and Protein G, which interact with the Fc region and bind exclusively to IgG heavy chains.

Protein L does not bind bovine immunoglobulins, which are present in the fetal bovine serum (FBS), and thus provides a convenient way to purify κ light chain-containing monoclonal antibodies from culture supernatant.

Binding capacity 

• 20-30 mg of human IgA/IgG per ml of gel