Protein L / Agarose
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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Protein L / Agarose Agarose for IgA and IgG purification - k light chain specific |
Show product |
2 x 2 ml |
gel-protl-4
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Agarose for IgA and IgG purification - k light chain specific
Protein L binds specifically to the variable domain of Ig κ light chain, as a consequence Protein L has the capacity to purifiy κ light containing IgA antibodies.
It should be noted that protein L binding is restricted to specific subclasses of the kappa domain.
As Protein L recognizes κ light chains, protein L can bind to all classes of Ig, in contrast to Protein A and Protein G which interact with the Fc region and bind exclusively to IgG heavy chains.
Protein L does not bind bovine immunoglobulins which are present in the fetal bovine serum (FBS) and thus provides a convenient way to purify κ light chain-containing monoclonal antibodies from culture supernatant.
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Contents
2 x 2 ml of Protein L / Agarose is provided as a 50% v/v gel slurry in 20% v/v ethanol (total volume 8 ml)
Protein L / Agarose is shipped at room temperature.
Store at 4°C.
DO NOT FREEZE.
Description
Protein L is an immunoglobulin-binding protein expressed by the anaerobic species Peptostreptococcus magnus [1]. Protein L binds specifically to the variable domain of Ig kappa light chain without interfering with the antigen-binding site [2]. It binds strongly to human kappa light chain subclasses I, III and IV, and also to most kappa light chains of other species such as rat and mouse. As it recognizes kappa light chains of other chains, protein L can bind to all classes of Ig, in contrast to Protein A and Protein G which interact with the Fc region and bind exclusively to IgG heavy chains.
Protein L / Agarose from InvivoGen uses the recombinant form of protein L coupled to beads using a leak-resistant chemistry that provides a support with minimal nonspecific binding.
1. Björck L., 1988. Protein L. A novel bacterial cell wall protein with affinity for Ig L chains. J Immunol. 1988 Feb 15;140(4): 1194-7.
2. Nilson BH. et al., 1993. Purification of antibodies using protein L-binding framework structures in the light chain variable domain. J Immunol Methods. 164(1):33-40.